Transferrin and FOB Combo Rapid Test

Intended use

The Fecal Occult Blood & Transferrin Test (Colloidal Gold) is a qualitative test that detects human hemoglobin and transferrin in human fecal specimens. The test is a visual one step, in-vitro assay. It is intended for professional use to help diagnose gastrointestinal bleeding.

Test principle

The Fecal Occult Blood & Transferrin Test (Colloidal Gold) is a lateral flow chromatographic immunoassay for the qualitative detection of hemoglobin and /or Transferrin hemoglobinin human feces.The membrane is pre-coated with anti-transferrin antibody and anti- hemoglobin antibody on the test line region of the strip. During testing, the specimen with hemoglobin and/or transferrin reacts with the particle coated with anti-transferrin antibody or anti-hemoglobin antibody ,the mixture migrates upward on the membrane chromatographically by capillary action to react with anti-transferrin antibody and /or anti- hemoglobin antibody on the membrane and generate a colored line. The presence of red band in the testing area(H) and (T),indicates a positive result for hemoglobin and/or transferrin. While absence of red band in testing area(H) and /or (T), indicates hemoglobin and/or transferrin is negative result.To serve as a procedural control, a colored line will always appear in the control line region indicating that the proper volume of specimen has been added and membrane wicking has occurred.

Materials and Components

1. 20 test devices (cassettes), each sealed in a foil pouch.

2. Fecal sample tube including 2ml dilute buffer (0.85% NaCL) for each test. One package insert (instructions for use).

3.One package insert (instructions for use). 

4.Timer.

5.Fecal container.

Product Information

Specimen requirements

1.Collect sufficient quantity of feces.

2.Fecal samples should be collected in clean and dry containers (no preservatives or transport media).

3.Urines should be avoided to collect in the containers.

Test Procedure

1. Randomly pierce the fecal specimen with the swab or stick at least 5 different sites and put into the fecal collection tubes with buffer.

2. Shake the fecal collection tube vigorously to ensure a good liquid suspension.

3. Remove the test cassette from the sealed foil pouch and place it on a flat surface.

4. Dispense 3 or 4 drops processed samples into each sample well on the test (S).

5. Read the result within 10 minutes.Do not interpret results after 10 minutes.